Journal: Nanomedicine : nanotechnology, biology, and medicine
Article Title: Evaluation of an adjuvanted hydrogel-based pDNA nanoparticulate vaccine for rabies prevention and immunocontraception
doi: 10.1016/j.nano.2019.102049
Figure Lengend Snippet: The activation of CD8 and CD62L positive cells in both splenocytes (top) and lymph node cells (bottom) were evaluated after re-stimulation with antigen. Representative flow cytometry dot plots for each group are shown in the figure above. Bar graphs plotted in figure 9A and andBB showed the CD8 positive cells in splenocytes and lymphnode cells. p value was calculated (*p<0.05 significant, **p<0.01 very significant, ***p<0.001 extremely significant) using one-way Anova, multiple comparison was performed using tukey’s post hoc analysis. Data are expressed as mean ± SEM.
Article Snippet: Antibodies used to stain cells for MHC I, MHC II, IFN-Υ, IL-4, CD45R, CD62L, CD4 and CD8 for flow cytometry analysis were purchased from eBioscience laboratories (San Diego, CA, USA).
Techniques: Activation Assay, Flow Cytometry, Comparison